modeling in physiology Protein kinetics determined in vivo with a multiple-tracer, single-sample protocol: application to lactase synthesis
نویسندگان
چکیده
MARY A. DUDLEY,1 DOUGLAS G. BURRIN,1 LINDA J. WYKES,2 GIANNA TOFFOLO,3 CLAUDIO COBELLI,3 BUFORD L. NICHOLS,1 JUDY ROSENBERGER,1 FAROOK JAHOOR,1 AND PETER J. REEDS1 1United States Department of Agriculture/Agricultural Research Service Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine and Texas Children’s Hospital, Houston, Texas 77030; 2School of Dietetics and Human Nutrition, McGill University, Montreal, Quebec, Canada H9X 3V9; and 3Department of Electronics and Informatics, University of Padua, Padua, Italy 35131
منابع مشابه
Protein kinetics determined in vivo with a multiple-tracer, single-sample protocol: application to lactase synthesis.
Precise analysis of the kinetics of protein/enzyme turnover in vivo has been hampered by the need to obtain multiple tissue samples at different times during the course of a continuous tracer infusion. We hypothesized that the problem could be overcome by using an overlapping (i.e., staggered) infusion of multiple stable amino acid isotopomers, which would take the place of multiple tissue samp...
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We have estimated the synthesis rates in vivo of precursor and brush-border (BB) polypeptides of lactase phlorhizin hydrolase (LPH) in newborn pigs fed with water or colostrum for 24h post partum. At the end of the feeding period, piglets were anaesthetized and infused intravenously for 3h with L-[4-3H]- phenylalanine. Blood and jejunal samples were collected at timed intervals. The precursor a...
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تاریخ انتشار 1998